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We then performed metagenomic analysis on exactly the same samples to locate the presence of reads from pks+ genes. As demonstrated in Fig. 3, the pks+ genes may very well be detected inside the sequencing data with the samples (either cancers or normal) in 19 sufferers from 30, Consequently validating the presence of your mutational and indel signatures (see also Supplementary Figs. 6 and 7). Notably, Whilst the presence of pks+ genes while in the sample signifies the existence of your genotoxic E. coli strands, the mutational signature is the result of generally earlier exposure, for this reason it could be accrued in mobile genomes previously devoid of pks+ getting current for the time being of sampling.
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